Wednesday, May 6, 2020
Autism Spectrum Disorder ( Asd ) - 1441 Words
Introduction Autism spectrum disorder (ASD) is a hereditary neurodevelopmental disorder. Like many other psychological disorders, it is commonly misunderstood in popular culture. Many allude to it casually, often equating it with crippling social awkwardness and disability when it is in fact a spectrum disorder with many degrees of severity. It is somewhat of an enigma within the scientific community as well; there are many things we do not know about ASD and there is ongoing research to learn more about the disorder. The purpose of this paper is to explain what is currently known about ASD: general facts, symptoms, prognosis, treatment, and demographics. A Description of Autism Autism is a highly heritable neurodevelopmental disorder. Itâ⬠¦show more contentâ⬠¦Structural and functional brain imaging reveals that ASD affects multiple parts of the brain (Amaral, Geschwind, Dawson, 2011), likely affecting the cerebellum, frontal cortex, hippocampus, and amygdala (Santangelo Tsatsanis, 2005). Many things are unknown about autism and scientists are searching for the causes of autism, the source of savant skills in those with autism, the best ways to diagnose ASD, the best ways to treat ASD, and more (Amaral, Geschwind, Dawson, 2011). Symptoms of ASD Symptoms of ASD are divided into three categories: problems with social interaction, problems with speech and communication, and problems with behavior (Amaral, Geschwind, Dawson, 2011). In general, symptoms of autism decline as a person ages (Social, 2013). Problems with social interaction are mainly connected to difficulty establishing close relationships with others (Rutter, 1978). Those with ASD may exhibit disinterest in reciprocating communication or in interacting with others at all, for example not responding to another personââ¬â¢s greeting (Fernell, Eriksson, Gillberg, 2013). They may also act apathetically, not caring if another person such as a parent is feeling sad or angry (Amaral, Geschwind, Dawson, 2011). Examples of problems with speech and communication include difficulty with pronunciation, pronoun reversal, where the speaker uses incorrect pronouns in a phrase, and echolalia, the uncontrollable
All My Sons By Miller Essay Example For Students
All My Sons By Miller Essay In the play All My Sons, by Arthur Miller, the word father means the personification of goodness and infallibility to Chris Keller. There was a strong relationship between Chris and his father, Joe. Everything Joe had done in his life was for Chris. His entire factory was intended for Chris once he retired. Throughout the play there was question of Joes innocence in the death of 21 pilots, who were flying planes that had parts from Joes factory. Chris strongly believed that his father played no part in those deaths and that the blame lay solely on Joes partner, Herbert Deever. At the end of the play, Chris realization that his father was guilty brings about anger and then remorse, when Joe commits suicide due to his guilty conscience. Joe has done everything in his life for his sons. Once his elder son Larry died in the war, Joe devoted his life to his younger son Chris. KELLER. Because what the hell did I work for? Thats only for you, Chris, the whole shootin-match is for you Miller, 15. Many businesses are handed down from generation to generation. Joe felt his role in life was to build a business that his son could take over once he got too old to run it. His love and hope for Chris blinded Joes ability to make a thoughtful and rational decision regarding faulty parts from his factory. This resulted in the death of the pilots. Joe completely disregarded the lives that his parts would be affecting because all he cared about was his son. The love that Joe displayed to his son was returned in the love and respect that Chris had for his father. GEORGE. But you know him. You know in your heart Joe did it. CHRIS. Lower your voice or Ill throw you out of here Miller, 46. George Deevers confrontation with Chris about his fathers guilt demonstrated Chris belief that his father was not guilty of any crime. He refused to believe that his father played a role in shipping the defective parts to the government. This only reinforced the idea that there was a strong father son bond between Joe and Chris. The moral values that were instilled in Chris blocked him from believing that his father was capable of anything else but good. These values prove to be the eventual down fall of Joe. At the end of the play Chris idealism and his moral outrage came across powerfully as he realized that his father was guilty. The solid connection between father and son was broken. As Chris struggled to understand his fathers actions, Joe still felt he had done no wrong. JOE. Nothing is bigger than the family. Im his father and hes my son. These were poignant and sad words by Joe as it showed he still had not realized the scope of the disaster he had caused. After reading the letter from Larry on the day of his death, the symbolic understanding ofà the title finally comes across in dramatic sorrow when Joe fully accepts his responsibility. He feels the only recourse is to take his own life. CHRIS. Mother I didnt mean toMiller, 69. Chris felt that because he had pressured Joe into accepting his responsibility, to the universe and the people in it, he was to blame for Joes suicide. This remorse demonstrated that although Chris was deeply angered and saddened by his fathers guilt, he still loved him. Throughout the play, All My Sons, the theme of the family appeared on a number of levels. .u28f98b547fda49815ca61607115a8228 , .u28f98b547fda49815ca61607115a8228 .postImageUrl , .u28f98b547fda49815ca61607115a8228 .centered-text-area { min-height: 80px; position: relative; } .u28f98b547fda49815ca61607115a8228 , .u28f98b547fda49815ca61607115a8228:hover , .u28f98b547fda49815ca61607115a8228:visited , .u28f98b547fda49815ca61607115a8228:active { border:0!important; } .u28f98b547fda49815ca61607115a8228 .clearfix:after { content: ""; display: table; clear: both; } .u28f98b547fda49815ca61607115a8228 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u28f98b547fda49815ca61607115a8228:active , .u28f98b547fda49815ca61607115a8228:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u28f98b547fda49815ca61607115a8228 .centered-text-area { width: 100%; position: relative ; } .u28f98b547fda49815ca61607115a8228 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u28f98b547fda49815ca61607115a8228 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u28f98b547fda49815ca61607115a8228 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u28f98b547fda49815ca61607115a8228:hover .ctaButton { background-color: #34495E!important; } .u28f98b547fda49815ca61607115a8228 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u28f98b547fda49815ca61607115a8228 .u28f98b547fda49815ca61607115a8228-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u28f98b547fda49815ca61607115a8228:after { content: ""; display: block; clear: both; } READ: A revelation of love EssayChris and Joe demonstrated their dedication and love to one another through a strong bond. Joe proved this by doing everything in life, including committing a crime, for his son, Chris. Chris showed this bond by not believing in his fathers guilt even though the truth lay at the tip of his nose. Even after the realization that his father played a role in many deaths Chris over came his anger and recognized that he still loved his father by demonstrating remorse at his fathers death. The tie between Joe and Chris Keller lay deep in the love and respect that each had for the other and proved to tough to break.
Thursday, April 23, 2020
Speak and the Breadwinner Coming of Age in Literature Essay Example
Speak and the Breadwinner: Coming of Age in Literature Paper There comes a time in everyoneââ¬â¢s life when they reach the point where they are no longer children, but adults. The transition from a child into a young adult is referred to as ââ¬Å"the coming of age,â⬠or simply growing up. Certain children reach this stage through a tragic, painful event, which sometimes can potentially change them and the way they view the world. Other children reach this stage by simply growing up and understanding everything around them. This stage in life is one of the most important in literature. The coming of age theme is found in many pieces of literature, such as Laurie Halse Andersonââ¬â¢s Speak and Deborah Ellisââ¬â¢ The Breadwinner. The main characters in both of these young adult novels experience growth and change in their own ways. In Laurie Halse Andersonââ¬â¢s Speak, readers are given a mental picture of who they think Melinda Sordino is. ââ¬Å"The bus picks up students in groups of four or fiveâ⬠¦I close my eyes. This is what Iââ¬â¢ve been dreading. As we leave the last stop, I am the only person sitting aloneâ⬠(3). What the reader gets is this picture of Melinda Sordino predicting that sheââ¬â¢s going to have the worst freshman year ever. We will write a custom essay sample on Speak and the Breadwinner: Coming of Age in Literature specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Speak and the Breadwinner: Coming of Age in Literature specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Speak and the Breadwinner: Coming of Age in Literature specifically for you FOR ONLY $16.38 $13.9/page Hire Writer What I think a lot of readers donââ¬â¢t pick up on right away is that this is the beginning of Melindaââ¬â¢s character developing. Speak is a story truly based on the coming of age theme, and unfortunately Melindaââ¬â¢s character grows as a result of a tragic event. The summer before freshman year, Melinda was living a normal, good life. She had tons of friends, good grades, and a loving family. Then the best night of her life turned into the worst. ââ¬Å"We were on the ground. When did that happen? ââ¬Å"No. No I do not like this. â⬠ââ¬Å"In my head, my voice is as clear as a bell: NO I DONââ¬â¢T WANT TO! (â⬠¦And he hurts me hurts me hurts me and gets up and zips his jeans, and smilesâ⬠(135-136). This traumatic experience would forever change Melinda. ââ¬Å"The novelââ¬â¢s title is Speak, but the silence of the main character predominatesâ⬠(Smith). Melindaââ¬â¢s inability to speak out about what happened isnââ¬â¢t uncommon in girls her age. ââ¬Å"The sadness, anger, and sense of betrayal that girls often feel entering this transitional period in their lives is increased by this shocking and demeaning experienceâ⬠(Smith). She couldnââ¬â¢t find it in her to tell anyone about what had happened, not even her parents. She just allowed it to take over her. She didnââ¬â¢t care what she looked like when she went to school. She didnââ¬â¢t have any friends because she didnââ¬â¢t have much to say. She came off and depressed, and thatââ¬â¢s just what she was. One girl befriended her, only to tell her a few weeks later that they couldnââ¬â¢t be friends anymore; she wasnââ¬â¢t giving her a good image. She was using her for her lack of friends herself. Melinda found herself in art. This was the one place she could express how she was feeling without anybody finding out the truth. Mr. Freeman, the art teacher at her high school, is the one person who helps her grow. He encourages her to express herself through her art theme: trees. This tree serves as a symbol in the book. All throughout the book Melinda struggles with life, just as she struggles with creating the perfect piece of art through trees. Mr. Freeman sees her pain through what she creates, and only encourages her more to keep going. The trees are symbolic of Melindaââ¬â¢s growth as a person. As she starts to realize that the rape was not her fault, her trees start becoming more real. Towards the end of the book Melinda asks her father to pick her up some flower seeds while he runs to the hardware store. This is one of the first steps towards communication. Through her whole isolated freshman year, Melinda finally finds the courage to speak out about what happened to her. It took the help of a classmate and an art teacher to help her realize that what happened was not her fault, and that she couldnââ¬â¢t live like that forever. When she shows Mr. Freeman her final tree, it is complete with wounds and a low dead branch. ââ¬Å"He sits on the stool and hands back my tree. You get an A+. You worked hard on this. Youââ¬â¢ve been through a lot, havenââ¬â¢t you? â⬠ââ¬Å"Let me tell you about itâ⬠(198). This is a major point in this book. Melinda is finally ready to speak. The low dead branch symbolizes who she was for that whole year; dead inside. After getting the truth out, Melinda begins to rekindle old friendships and communication with her par ents. Melinda has grown so much through this experience. She has learned that one must speak up for themselves. Our society needs to instill this is children at a young age. It seems to be something that we struggle with. She has matured so much since the beginning of the school year. In Deborah Ellisââ¬â¢ The Breadwinner, Parvana, the main character, experiences much of the coming of age theme through a traumatic and painful experience. Being a female in Afghanistan is not easy. Their freedom has been taken away from them. They are not allowed to attend school. They are not allowed to be outside, unless they are with their father or husband. Freedom has been stripped from their lives. Their lives were pretty bad, but at least their family was together. Little did they know it was about to get worse. The whole family was laughing when four Taliban soldiers burst through the doorâ⬠¦Two soldiers grabbed her fatherâ⬠¦Parvana watched helplessly as two soldiers dragged him down the steps, his beautiful shalwar kameez ripping on the rough cement. Then they turned a corner, and she could see them no moreâ⬠(30-31). Being a child was no longer an option for Parvana. She needed to grow up fast. Parvana was elected to do the unthinkable-she was about to become a boy. After all, she was the least developed. Her older sister Nooria was too developed to take on that role. This is definitely one example of the coming of age theme. Parvana has stepped up and has taken the role of ââ¬Å"Kaseem. â⬠This was the only way to provide for her family. Stepping out of her gender was the only option, the only way they wouldnââ¬â¢t get caught and punished, or even killed. Parvana found a job reading letters sent to the soldiers in the Taliban. She made enough to barely feed her family, but it was better than nothing. She soon ran into a girl from one of her classes when she was able to attend school, who had the same idea that Parvanaââ¬â¢s family had for her. She was a ââ¬Å"tea boyâ⬠now, trying to provide for her family also. She came up with an idea that she knew made more money than what they were making. They were going to dig up bones. Nooria, Parvanaââ¬â¢s older sister, was never really nice to her, and was always bossing her around. This could just be their age, and not completely understanding how severe their situation really is. Nooriaââ¬â¢s dream was to go to college, and that was taken away from her. This may have left Nooria bitter. When Parvanaââ¬â¢s mother learns of her new way of making money, she is absolutely against it. ââ¬Å"So this is what weââ¬â¢ve become in Afghanistan,â⬠Mother said. We dig up the bones of our ancestors in order to feed our familiesâ⬠(115). Nooria then sticks up for Parvana. This is the first time Nooria has been positive about anything Parvana has done. Nooria has matured through this whole experience. She realizes how important family is, and that desperate times call for desperate measures. ââ¬Å"These are unusual times. They call for ord inary people to do unusual things, just to get byâ⬠(116). Parvana and her friend did this long enough to earn a decent amount of money, and Parvana went back to her blanket in the market. Parvana really stepped up for her family. She took her siblings outside whenever they had the chance, she worked so she could go to the market and buy food for her family, and she was constantly getting water from the neighborhood tap. She realized that she could no longer be passive, and that she had to take control. And that is just what she did. Parvana finally got her father back, but now her mother and sister were on their way to Mazar, where they thought they would be safe. They soon found out that the Taliban was there, also. Though the ending isnââ¬â¢t exactly happy, it does offer hope that the family will be reunited The novelââ¬â¢s focus on how womenââ¬â¢s lives are heavily restricted by laws rather than by personal choice is crucial in understanding the nature of external freedomâ⬠(Harper). Parvana and her family want to fight for freedom, and her mother demonstrates that nicely. Her and a friend create an underground magazine that would be sent to women all over the world. This family is truly rema rkable. So much maturing and growing existed in this family. Both Speak and The Breadwinner demonstrate the coming of age theme. The characterââ¬â¢s growths in these stories are just phenomenal. Sometimes it takes a really bad situation to truly learn about oneââ¬â¢s self. Both Melinda and Parvana had to experience something traumatic in order to find who they truly were. Parvana learned that she would do anything for her family, even her bossy sister. Melinda learned the importance of speaking up and standing up for herself. These books teach valuable life lessons. Not only is The Breadwinner a coming of age novel, it teaches about what life is like in Afghanistan. It reminds readers how lucky they are to be living in a country where they have so much freedom. Incorporating vocabulary used in Afghanistan allows readers to really feel like they are in the story. Coming of age in literature truly allows readers to relate to characters in the story, and aide them in growing. Works Cited Anderson, Laurie Halse. Speak. New York: Penguin Group, 1999. Bean, Thomas W. and Harper, Helen J. Exploring notions of freedom in and through young adult literature. Journal of Adolescent Adult Literacy, Oct2006, Vol. 50, Issue 2, p96-104, 9p (AN 22521106). Ellis, Deborah. The Breadwinner. USA: 2001. Smith, Sally. Journal of Adolescent Adult Literacy, 10813004, Mar2000, Vol. 43, Issue 6.
Tuesday, March 17, 2020
buy custom Anorexia Nervosa essay
buy custom Anorexia Nervosa essay Eating Disorder An eating disorder is a type of illness that is characterized by eating behaviors that deviate from the normal eating habits and is usually a response to a psychological or emotional need of self criticism and negative thoughts of oneself. In trying to satisfy these needs, a person develops certain rituals about food, weight, and body shape leading to indulgence in over eating or refusing to eat by ignoring normal body signals for example not eating even when hungry or consuming excess food even when the person is satisfied. Abnormal eating patterns which include anorexia nervosa, bulimia nervosa and binge eating are the three major types of disorders. Anorexia Nervosa Anorexia nervosa is a type of eating isorder where a person has an extreme weight phobia and has an obsession to remain being thin always. Due to this fear, people with anorexia strive to keep their body weight to the low by self starvation or refusing to eat, self-induced vomiting, fasting or by doing excessive exercises and abuse of diet aids. Although there are few cases affecting men, this disorder is usually common with young women starting usually at the adolescence stage or early childhood. Causes of anorexia are not clearly known although certain mixed causes involving psychological factors, social factors, age, stress levels, family and genetic influence are believed to take role. Some of the symptoms of anorexia include intense weight phobia, desire to lose weight despite being thin, constant awareness of their weight, excessive exercises and keeping strict routines of them, certain food choices like salads and for women, lack menstrual periods. Some of the effects of anorexia may include malnutrition, emaciation, hair loss, low blood pressure, organ failures, bone thinning, brain damage, infertility and even death. Treatment of anorexia has not been uniquely identified although many of the forms of treatment include medical supervision, psychotherapy and nutrition therapy. An attempt to restore weight to a healthy and normal size is the key component to any form of treatment but some extreme cases of weight loss may require hospitalization. The use of some medicines such as antidepressants, mood stabilizers and antipsychotics has also been thought to treat anorexia. A better form of treatment involving a combination of supervised medical attention and supportive psychotherapy is also currently being proposed and is thought to be effective than therapy alone. Buy custom Anorexia Nervosa essay
Saturday, February 29, 2020
Analysis of Linear DNA Genomes Separation in Gel Electrophoresis
Agarose gel electrophoresis has been widely used as a form of separating DNA genomes in varying sizes from 100 kp upto 25 kb. Isolation of Agarose gel is obtained from the genera Gelidium and Gracilaria.in the gelato process, the polymers of agarose often form an association of none covalent which form networks of pore sizes which determine the molecular ability of sieving properties. Use of gel electrophoresis is beneficial in separation of DNA genomes. Electrophoresis process is key in separating the different nucleic acids using various sizes and charges depending on the contents of the solution. In this experiment, lab analysis of gel was used to put gel solutions in charged nucleic acids for separation purposes. At this point the larger DNA and RNA have a hard time in separating thus allowing time for separation of the genomes based on the sizes. The rate of separation of the DNA molecule in the experiment was determined by the rate at which the sizes of the DNA, the concentration of the gel, DNA Conformation present, voltage degree applied, ehidium bromide solution introduced, type of agarose and the buffer being utilized in electrophoresis. After the process of separation, DNA molecules will be able to be visualized in the UV light using staining process to identify the different genomes. Thus in essence DAN electrophoresis defines the process by which the DNA migrates in the supporting medium. Most of electrophoresis is carried in agarose gels in narrow polymers of gels using pores of different sizes, this sieving provides a means by which the pores gives an opportunity for the DNA molecules to go through the pores at different sizes thus being separated using molecular weights. Thus this laboratory report uses agarose Gels while staining with ethidium bromideà to assess the separation process of the different DNA genomes. Thus it seeks to investigate the DNA genome separation to assess the different nucleic acids by their respective sizes. Refer to the Lab Manual 5 for in-depth methodology and procedure. Diagrammatic presentation of gel DNA Table 1; Showing gel electrophoresis picture Table 2; Showing curve presentation of the base pairs against distance travelled Table 3; Showing table figure for the curve Table 4; Showing how to calculate base pairs Example suppose we have a base pair having travelled 0.3 cm, then draw a line as illustrated above and take the readings on the corresponding logbp and take the anti log, which you get the base pair size. Table 5; Showing the sizes of pUC19 and their insert sizes Agarose gel electrophoresis has been utilised as a common method for separation of proteins, (Kryndushkin et al., 2003). The basic forms of nucleic acids can be separated through the aid of electrification process whereby charged molecules move to the anode side. This migration as depicted in the experiment ensures that molecules which have lower molecular weight are able to move faster, (Sambrook & Russel 2001). The process of electrophoresis is a crucial step in ensuring purification process of the desired DNA bands. In this experiment the usage of ethidium bromide is essential in visualizing the staining of the transcend DNA molecules. In this task, the Agarose gel electrophoresis plays a key role in ensuring the characteristics of DNA are obtained without any alterations. This experiment has yielded results which have enabled determination of DNA fragments sizes through digestion by restriction enzymes. The visualization has been effected with the use of ethidium bromide which is a common agent in nucleic acid purification process. The Agarose gel concentration on this task entailed the separation of the gel using agarose gel concentration of 0.2%w/v having bands from 0.1-1 kb. The distance travelled by DNA molecules in electrophoresis is directly proportional to the size of the DNA itself. The agarose gel is beneficial in ensuring that there are movements based on their sizes. With the various differences between the various rates of the DNA molecules in the gel solution, they are separated based on the size of the bases. The relationship built between the varied sizes of the DNA genome. The sieving of DNA is done through the size which it bears, (Southern, 1975). The length of DNA strands often vary from 50 base pairs to upto million s base pairs which agarose gel electrophoresis can be effective in separating them , the migration and distance travelled is linked on the concentration of the agarose used to prepare the gel. Concentrations having lower concentration are able to travel faster in the distance travelled and vice versa. In this study agarose gel of 2% has been used which was effective in separating the DNA at range of 0.1-1 kb, the low percentile gels often signify gels which are weak. Double stranded DNA moves faster as the molecules travels; its speed is inversely proportional to the logarithm of base pairs. This linked and established relationships depends on the strength of the of gel composition. The distance travelled by the digested genome signifies that there is action of restriction enzymes which shows that there restrictions which have taken place, thus distinguishing the variability linked to genetics and enzyme cost. The digested fragments were this separated using the agarose gel electrophoresis which showed continuous smear on the gel surface with the distribution of the difference fragment sizes being established. Digested pUC19 is a plasmid and able to transform itself on the transformation process where it can be able to multiply itself and express. Undigested pUC19 originate from E coli and contain high number of base pairs. The transformation efficiently portrayed shows that smaller pUC19 plasmid sin E choli can be manipulated and be transformed from the ampicilin forms. This shows that the DNA is in contact form with plasmid DNA being intact and with presence of viral chromosomes which can be transformed into high efficiencies. This transformation is through the resulting effect of digestion of peri plasmids. The undigested Puc19 shows presence base pairs which have the ability to perform recombination and be incorporated into cells, (Goto, Kenta & Yukio, 2013). The lanes which have recombination factor is able to facilitate the cloning of DNA in host cells. à This signifies recombination of various fragments of gel solution. The lanes that have been generated originated from digestion of particular DNA, which gives it equimolar amounts. Based on the lanes, there is variation on the number of non molar amounts, thus signifying that there is difference in band lengths. Others have shown to represent circular forms of the plasmids which is dependent on the age and quality of the plasmids. The existence of three forms of DNA formation which exists include linear formation, open circular formation and supercoiled forms. Plasmid DNA have been prevalently been studied in laboratory studies. After its preparation they exists in the three forms above. With good plasmid preparation, DNA often form plasmid which exist in any one strands of the DNA, this break causes the release of the phosphordiester backbones of the DNA to be released out. The visualising process of the agarose gel using the standard control tool is key to assess whether the bands have created a generation or not. Closer bands are well compressed than far away bands as indicated in the gel view. The standard marker used in this experiment was essential in ensuring that the standards sizes are generated using base pairs. This result signifies that electrophoresis is an effective way of separating nucleic acids. High gel agarose gives room for handling of low percentage gel separation. Due to the size of the base pair present in this experiment, has utilised field gel electrophoresis. This isà comparable to studies done (Lee et al, 2012), which have shown that sizes of DNA can be separated effectively through plotting on the log of molecular weight and different bands of DNA against the distance moved, this portray how different forms of gel can be able to move at different speeds. Super coiled plasmid DNA have sown to move faster, while those in linear formation travel averagely while open circular travel slowly. Goto, K., & Nagano, Y. (2013). Ultra-low background DNA cloning system. PloS one, 8(2), e56530. Kryndushkin DS, Alexandrov IM, Ter-Avanesyan MD & Kushnirov VV (2003). Yeast [PSI+] à prion aggregates are formed by small Sup35 polymers fragmented by Hsp10. Journal of Biological Chemistry.278 (49): 49636. Lee, P. Y., Costumbrado, J., Hsu, C. Y., & Kim, Y. H. (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of visualized experiments: JoVE, (62). Sambrook J&Russel DW(2001). Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY. Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J mol biol, 98(3), 503-517.
Thursday, February 13, 2020
Chronic Eczema Essay Example | Topics and Well Written Essays - 250 words
Chronic Eczema - Essay Example For instance, the methods of treating Chronic Eczema may change, hence rendering the previous routines useless. For example, a new method of diagnosing Chronic Eczema may be discovered, and hence taking of picture of the affected part by the patient and sending it to the Dermatologist becomes less useful. The technologies available to different patients are varied. The knowledge to make proper use of the technologies available to the patient are also not guaranteed. For instance, relying on the photos taken by the patient may not give a clear picture of the intensity of the infection, especially if the focus of the photos is not good. For this reason, relying on the patientââ¬â¢s technologies may impair the process of offering equitable health services. For equitability in health service delivery, practices such as diagnosis should be uniform across the patients. The virtual support hinders the patient from accessing quality services, which would be offered in the event of the patient visiting the GP and hospitals. It is likely that if the patient gets virtual support, he/ she doesnââ¬â¢t enjoy some of the services, which are difficult to implement in the online support
Saturday, February 1, 2020
Assignment Example | Topics and Well Written Essays - 500 words - 214
Assignment Example I believe that this is the sign that he was extremely talented. As the presented puts it, every next text that was writer afterward simply expanded the ides which were created in his two fundamental works. The first one is called The World as Will and Representation and the other one is called On the Fourfold Root of the Principle of Sufficient Reason. Another aspect of his life that really influenced me as the decision that he made when he was young. It is said that he could pursue many different careers, including running a business, but he knew that his accomplishments would lie in the academic field. Having learned about the idea which were develop by this philosopher, I must admit that he is truly one of the most wise scholars of his century and the science would have been greatly damaged in case Schopenhauer had chosen a different field of activity. It was suggested in the video that he was able to influence a considerable number of people who were later recognized as prominent individuals as well. It is quite remarkable to see the way he treated the concept of will and tried to use it in order to explain the world as it is. I believe that a careful examination of his legacy is beneficial to all
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